The full total results showed that beneath the infection ofEscherichia coliandCandida albicans, vaginal epithelial tissue showed hyperplasia and edema, a lot of epithelial cells were shed, and a lot of inflammatory cells were infiltrated. make it near to the regular group and enhance the broken genital epithelial tissues. The outcomes of ELISA demonstrated thatBCFYQdecreased the degrees of IL-1 and IL-2 and elevated the degrees of IL-13 and IgA (P<0.05). Furthermore,BCFYQmay raise the plethora of vaginal flora, especiallyLactobacillus. The differential metabolite enrichment pathway suggests that the therapeutic mechanism ofBCFYQis mainly related to lipid metabolism and amino acid metabolism. == Conclusion == Our research shows thatBCFYQhas a good therapeutic effect on mixed vaginitis. It repairs the damaged Rabbit Polyclonal to PRKAG1/2/3 vaginal mucosa by regulating the vaginal flora and lipid metabolism disorders to improve the local immune function of the vagina and inhibit the growth and reproduction of pathogens. Keywords:Chinese medicine suppository, mixed vaginitis, vaginal flora, Lactobacillus, lipid metabolism == Introduction == Vaginitis is a common and diverse gynecological disease. It is a general term for a variety of vaginal mucosal inflammatory diseases caused by different pathogens invading the vagina. It is mainly divided into aerobic vaginosis (AV), bacterial vaginosis (BV), trichomonad BAPTA tetrapotassium vaginitis(TV) and Vulvovaginal Candidiasis (VVC). Mixed vaginitis is the infection of the vagina by BAPTA tetrapotassium at least two different pathogens at the same time, both of which contribute to an abnormal vaginal environment leading to signs and symptoms (Tumietto et al., 2019). Clinically more frequent mixed infection with bacteria and Candida albicans BAPTA tetrapotassium (Benyas and Sobel, 2022). Vaginitis, if not treated in time, leads to a decline in a womans local immune function and subsequent infection with other gynecological diseases such as cervicitis, pelvic inflammatory disease, cervical erosion, etc. Currently, antibiotics are the main treatment for vaginitis. However, they also kill the symbiotic bacteria, leading to an imbalance of the vaginal flora and also to antibiotic resistance of pathogens (Stojanovi et al., 2012). Therefore, the search for effective alternative therapies with few side effects has become a new field of research. The female vaginal microecology is one of the most important microecological systems in the human body, and it is directly or indirectly related to various gynecological diseases such as vaginitis. More and more researchers are advocating the treatment of vaginitis by improving the vaginal microecology. In recent years, probiotics used to combat vaginal dysbiosis have shown great promise in restoring normal vaginal flora, including preventing the loss of beneficial BAPTA tetrapotassium bacteria (Das et al., 2022). Therefore, in this study, vaginal microbial flora was used as a vital indicator, and high-throughput BAPTA tetrapotassium sequencing of 16S rRNA gene to investigate whetherBCFYQcould treat vaginitis by improving vaginal microecology. Traditional Chinese medicine suppositories for the treatment of vaginitis first appeared in the Western Han Dynasty in China. Studies have shown that traditional Chinese medicine suppositories have unique effects in the treatment of various gynecological inflammations, have fewer side effects, do not easily to cause drug resistance, and have advantages in restoring normal physiological functions of patients and eliminating potential causes (Ma et al., 2017).BCFYQis a traditional Chinese medicine suppository developed from traditional Miao ethnomedicine (registration number: Z20026597). Its main ingredients areSophora flavescens(0.64g),Stemona japonica(0.32g),Cnidium monnieri(L.) Cuss. (0.32 g),Callicarpa macrophylla(0.32g), Agrimonia Pilosa(0.32g), Alum (0.01 g), Borneol (0.01 g), Camphor (0.06 g), and Boric acid (0.05 g).Sophora flavescenshas been used for thousands of years to prevent and treat common ailments such as eczema, diarrhoea, vaginal itching and colitis (He et al., 2015). Studies have shown that the main active ingredient ofStemona japonicais alkaloid, which has the functions of anthelmintic, insecticidal, antitussive and anti-asthmatic, antitumor and antibacterial (But et al., 2012). Recent studies have shown thatCnidium monnieri (L.) Cuss. has a variety of pharmacological activities, mainly in anti-inflammatory, antioxidation and antibacterial itching (Tsai et al., 2015; Z.Wang and Shen, 2017).Callicarpa macrophyllahas a variety of biological activities such as analgesic, anti-inflammatory, antipyretic, antifungal, antiarthritic, and antitumor (Chung et al., 2014;Feng et al., 2017; Z. H.Wang et al., 2019).Agrimonia pilosahas a long history of medicinal use and is also widely used for gynecological disorders (Y. M.Lee et al., 2012). TIAN Wen-yangs review summarised the clinical studies on BCFYQ in the treatment of vaginitis, among which there were 2 literatures on the use of BCFYQ alone in the treatment.