Slides were blocked with 1% BSA in TBS\T (20?mM TrisCHCl, 150?mM NaCl, 0.1% Tween\20, pH 8.0), incubated with 5 then? g/ml of Siglec\Fc in 4C
Slides were blocked with 1% BSA in TBS\T (20?mM TrisCHCl, 150?mM NaCl, 0.1% Tween\20, pH 8.0), incubated with 5 then? g/ml of Siglec\Fc in 4C