Because of this, the risk of transgressions in coeliac patients adhering to a gluten-free diet increases between 32% and 55% [27,28]. short arm of chromosome group 1, common LMW-GS are coded by genes at loci, genetically linked to loci, and finally, HMW-GS are coded by genes at loci present around the long arm of chromosome group 1 (Determine 2). The presence of gliadins and glutenins, and the balance between these two types of proteins is essential for the quality of the final product . Open in a separate window Physique 1 Gliadins and glutenins fractions revealed by using acid polyacrylamide gel electrophoresis (A-PAGE) and sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE), respectively (A) and RP-HPLC (B). , -gliadins portion; , -gliadins portion; /, /-gliadins portion; HMW, high molecular excess weight glutenins subunit; LMW, low Sucralfate molecular excess weight glutenins subunit. Open in a separate windows Physique 2 Gliadins and glutenins genes location in wheat chromosomes 1 and 6. Reprinted from thesis manuscript of Gil-Humanes (https://helvia.uco.es/handle/10396/5233). 2. Wheat Pathologies and Gluten-Free Diet (GFD) Wheat is usually associated with pathologies such as coeliac disease (CD), which affects about 1% of the population worldwide , non-coeliac wheat sensitivity (NCWS)  and allergies; bakers asthma  and wheat-dependent exercise-induced anaphylaxis (WDEIA) . Bakers asthma is usually a respiratory allergy brought on by a wide range of wheat proteins that react with immunoglobulin E (IgE). Wheat proteins responsible for bakers asthma comprise gliadins, glutenins, serine proteinase inhibitors (serpins), thioredoxin, agglutinin and several enzymes . The -amylase inhibitors are included among these enzymes, which are proteins soluble in chloroform: methanol (CM-like proteins) . These -amylase inhibitors have been described as the major group of proteins responsible Sucralfate for this allergy. Wheat is also responsible for WDEIA, which is an allergic reaction caused by combining the ingestion of wheat food and subsequent physical exercise. The major allergens associated with WDEIA are the -5 gliadins [9,11]. Palosuo et al.  suggested that this activation of transglutaminase in the intestinal mucosa could be provoked by the development of large allergen complexes responsible for triggering anaphylactic reactions during physical exercise in patients with WDEIA. NCWS is usually a common and heterogeneous pathology. The disease has been described as a reaction to Sucralfate gluten proteins, in which allergic and autoimmune mechanisms have been excluded. In fact, other proteins LAMB3 antibody Sucralfate such as metabolic proteins called -amylase/trypsin inhibitors (ATI) , and FODMAPS (fermentable oligo-saccharides, disaccharides, monosaccharides and polyols) seem likely candidates to cause this pathology. Removing gluten from the diet is the only way to normalise the small-bowel mucosa as well as improving the symptoms. On the other hand, you will find conflicting results about the presence of a wheat/gluten-induced inflammation in the majority of patients, as the mucosa from patients with gluten/wheat sensitivity does not express markers of inflammation, and their basophils are not activated by gliadin . Coeliac disease is the most analyzed of these pathologies. It is an autoimmune disorder that occurs in genetically predisposed individuals brought on by gluten proteins from wheat (gliadins and glutenins), rye (secalins), barley (hordeins), oats (avenins), and also, all hybrids in which any of the harmful cereals are involved. CD has a strong environmental component, gluten, but also a genetic component, concerning the human leukocyte antigen (HLA)-DQ2 and HLA-DQ8 . Gluten proteins are characterised by a high content of proline and glutamine residues making their total digestion hard. This gluten composition produces large peptides with immunostimulatory activity in the intestinal lumen . These immunogenic peptides cross the intestinal epithelium and are deamidated by the tissue Sucralfate transglutaminase 2 (tTG2) in the lamina propria , providing a negative charge to gliadin peptides and hence enhancing their affinity to bind HLA-DQ2/8. It causes the activation of CD4+, triggering intestinal damage and malabsorption symptoms. For immunological reasons, 95% of.